Chemically Functionalized Water-Soluble Single-Walled Carbon Nanotubes Obstruct Vesicular/Plasmalemmal Recycling in Astrocytes Down-Stream of Calcium Ions

Chemically Functionalized Water-Soluble Single-Walled Carbon Nanotubes Obstruct Vesicular/Plasmalemmal Recycling in Astrocytes Down-Stream of Calcium Ions

Blog Article

We used single-walled carbon nanotubes chemically functionalized with polyethylene glycol (SWCNT-PEG) to assess the effects of this nanomaterial on astrocytic endocytosis and exocytosis.We observed that the SWCNT-PEG do not affect the adenosine triphosphate (ATP)-evoked Hardware Ca2+ elevations in astrocytes but significantly reduce the Ca2+-dependent glutamate release.There was a significant decrease in the endocytic load of the recycling dye during constitutive and ATP-evoked recycling.

Furthermore, SWCNT-PEG hampered ATP-evoked exocytotic release of the loaded recycling Tank dye.Thus, by functionally obstructing evoked vesicular recycling, SWCNT-PEG reduced glutamate release from astrocytes via regulated exocytosis.These effects implicate SWCNT-PEG as a modulator of Ca2+-dependent exocytosis in astrocytes downstream of Ca2+, likely at the level of vesicle fusion with/pinching off the plasma membrane.